Unravelling CD8+ T cell memory functional programming
Responsable du Stage : Gregoire Lauvau
Tél : 718 678 1188 E-mail: gregoire.lauvau@einsteinmed.org
Department of Microbiology and Immunology, Albert Einstein College of Medecine Bronx, NY, USA
Résumé du Projet de Stage
CD8+ T cells are the only cells that can effectively sense intracellular pathogens infected cells and tumor cells, to kill them. Most approved vaccines, however, rely upon antibodies (Ab) for protection, thus there is an absolute need for improving vaccines that induce effective memory CD8+ T cells. This can only be achieved through rationale understanding of how naive, antigen-inexperienced T cells receive and integrate priming signals to form memory. Seminal studies have established that a single naive CD8+ T cell can give rise to multiples progenies. While cognate antigen stimulation determines the extent of primary response and the size of the memory cell pool, it is believed that costimulation and cytokines largely modulate the fate of naïve CD8+ T cells towards effector versus memory cells. It is also well accepted that cognate antigen triggering does not lead to functionally distinct pools of memory cells. Here, however, and contrasting to this dogma, we discovered that the strength and stability of cognate antigen/MHC interactions with the T cell receptor (TCR) modulate the acquisition of distinct functional characteristics by memory CD8+ T cells through epigenetic imprinting. This project will focus on mechanistically defining the epigenetic modifications that account for specific memory CD8+ T cell functional programs and testing them in relevant mouse models. The work will be developing skills related to sequencing data analysis, lentiviral-based genetic modification of T cells, the use of various genetically modified mouse models and high dimensional flow cytometry.
Projet accueil M2-BMC-2021-2022 (Lauvau)
Equipe d’Accueil : Stroma, inflammation & Tissue Repair
Intitulé de l’Unité : Department of Microbiology and Immunology, Albert Einstein College of Medecine Bronx, NY, USA
Nom du Responsable de l’Unité : Steven Porcelli, Professor and Chair
Nom du Responsable de l’Équipe : Gregoire Lauvau, Professor
Department of Microbiology and Immunology, Albert Einstein College of Medecine Bronx, NY, USA
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