Structure and function of a bacterial killing machinery and its toxins.


Responsable du Stage :GUBELLINI Francesca

Tél : +33 (0)1 40 61 39 84

Institut Pasteur


Résumé du Projet de Stage 

The M2 student will work in the Structural Microbiology Unit (Dir. Prof. P. Alzari) under the supervision of Dr. F. Gubellini on a project coupling cystallography and cryo-electron microscopy to investigate a mechanism of bacterial pathogenesis and competition. The Type 7 Secretion System is a large molecular machine inserted in the bacterial membranes. Discovered in Mycobacterium tuberculosis about 20 years ago, it was later identified in most Gram-positive bacteria. Bacteria use it to export different toxins attacking the host and/or killing other bacteria.
We use non-pathogenic bacteria (Bacillus subtilis and Escherichia coli) to purify these complexes and their toxins, studying their structure and their in vivo function. With this strategy we aim to understand bacterial communities of pathogenic and non pathogenic bacteria. The Master student will perform proteins mutagenesis and purification and characterize the structure of purified complexes by crystallography. During this internship the student will also participate in the Cryo-Electron Microscopy part of the project (samples preparation, screening, data collect and analysis).
The Structural Microbiology Unit is a dynamic environment in which students and researchers from different countries share their enthusiasm for science. The Institut Pasteur has state-of-the-art support for our research, including the NanoCore facility (cryo-EM), the Biophysics facility and the Crystallography platform.


Tassinari et al. doi:
Rapisarda et al. doi: 10.1146/annurev-micro-090817-062702
Gubellini et al. PMID: 28755367
Low*, Gubellini*, et al. doi: 10.1038/nature13081

Ce projet s’inscrit-il dans la perspective d’une thèse :

                                                       oui  x

                                                       non o

 si oui type de financement prévu : Ecole Doctorale

 Ecole Doctorale de rattachement : MTCI


Intitulé de l’Unité :Unité de Microbiologie Structurale, Institut Pasteur, UMR3528

Equipe d’Accueil :Unité de Microbiologie Structurale, Institut Pasteur, UMR3528

Nom du Responsable de l’Unité : ALZARI Pedro

Nom du Responsable de l’Équipe :GUBELLINI Francesca

Adresse : Institut Pasteur, 25 rue du Dr Roux, 75015 Paris