Equipe d’accueil : Normal and pathological hematopoiesis
Intitulé de l’unité : Institut Cochin
Nom du responsable de l’unité : Florence Niedergang
Nom du responsable d’équipe : Yannick SIMONI / Michaela FONTENAY
adresse : 22 rue Méchain, 75014 Paris
Responsable de l’encadrement : Nicolas DEREDEC
E-mail : nicolas.deredec@inserm.fr
Résumé du projet
Blood cancers originate from blood-forming tissues such as the bone marrow. They arise from disruptions in normal hematopoiesis, which leads to an uncontrolled proliferation of abnormal blood cells.
This M2 project aims to decipher in detail the anti-tumor T response in multiple myeloma and pediatric acute myeloid leukemia. Paired blood and bone marrow samples before and after treatment will by analyzed by using cutting edge methods for data acquisition and analysis.
The data will be acquired using a mass cytometer (CyTOF) allowing a multidimensional and simultaneous analysis of the immune populations over time. A panel of 30 surface markers will be used to characterize in depth the subpopulations of T lymphocytes (Tγδ, MAIT, Treg, conventional CD4 and CD8, etc.), with markers of activation (e.g. CD28, KI67…), inhibition (e.g. PD-1, CTLA-4, Tim-3…) and cytotoxicity (e.g. Perforin, Granzyme..). Coupled to the multiplex MHC class I tetramers assay, mass-cytometry will allow us to screen up to 200 tumor antigens for each patient, and characterize phenotypically tumor-specific CD8 T cells for each identified epitope using high dimensional data analysis. These data will be analyzed using computer programs developed on R (e.g. « clustering » of populations, statistical correlations, UMAP) (see Publication list for details).
Ce projet ne s’inscrit pas dans la perspective d’une thèse
Publications en lien avec le projet
Aziez L,…, Simoni Y Pro-inflammatory role of granzyme K producing bystander CD8 T cells in acute myeloid leukemia
Submitted, 2025
Simoni Y, …, Newell EW. Bystander CD8(+) T cells are abundant and phenotypically distinct in human tumour infiltrates.
Nature. 2018
Simoni Y, Chng MHY, Li S, Fehlings M and Newell EW Mass cytometry: a powerful tool for dissecting the immune landscape. Current Opinion in Immunology. 2018
Simoni Y, Fehlings M and Newell EW. Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry. Methods in Molecular Biology. 2019