Stromal regulation of tissue immunity and inflammation
Responsable du Stage : Lucie PEDUTO
Tél : 01 44 38 94 27 E-mail: firstname.lastname@example.org
Institut Pasteur- Département d’inmmunologie
Résumé du Projet de Stage
Stromal cells form the structural component of all our organs. In lymphoid organs, specific subsets of stromal cells organize the recruitment, localization, survival and interaction of immune cells and are therefore essential for the homeostasis of the immune system and for immune responses (Rodda, Immunity 2018). While stromal cells are major players in organ development and homeostasis, they are also strongly involved in development of chronic inflammatory diseases and autoimmune diseases (Croft, Nature 2019), even though the mechanisms remain unclear.
Our lab investigates the role of the stromal microenvironment in tissue homeostasis and disease pathogenesis, with a focus on inflammatory diseases and cancer (Dulauroy, Nature Medicine 2012; Stzepourginski, PNAS 2017; Di Carlo, JCI 2018; Benabid, Curr Opin Immunol 2020). In this project we aim at identifying novel mechanisms regulating tissue immunity and the inflammatory response by the stromal microenvironment. We will investigate the role of specific subsets of stromal cells in the regulation tissue immunity and inflammation, with a focus on mucosal/adipose tissues, using unique mice models, FACS and transcriptomics analysis. To assess the role of stromal cells in inflammatory diseases, cells will be depleted in vivo in mice models using a diphteria-mediated depletion approach, or using specific floxed mice models, and the impact on the immune response will be assessed by FACS or by measuring cytokines. The molecular mechanism will be investigated using transcriptomics and in vitro assays.
Publications de l’équipe relatives au projet de stage
- Benabid A, Peduto L. 2020. Mesenchymal perivascular cells in immunity and disease. Curr Opin Immunol 64 :50-55.
- Di Carlo S, Peduto L. 2018. The perivascular origin of pathological fibroblasts. J Clin Invest, 128(1):54-63.
- Stzepourginski I, Nigro G, Jacob JM, Dulauroy S, Sansonetti PJ, Eberl G, Peduto L. 2017. CD34+ mesenchymal cells are a major component of the intestinal stem cells niche at homeostasis and after injury. PNAS, 114(4): E506-E513.
- Dulauroy S, Di Carlo SE, Vives FL, Eberl G, and Peduto L. 2012. Lineage tracing and genetic ablation of ADAM12(+) perivascular cells identify a major source of profibrotic cells during acute tissue injury. Nature Medicine, 18(8): 1262-1270.
Ce projet s’inscrit-il dans la perspective d’une thèse :
ED d’appartenance : BIOSPC
Equipe d’Accueil : Stroma, inflammation & Tissue Repair
Intitulé de l’Unité : Stroma, inflammation & Tissue Repair
Nom du Responsable de l’Unité : Lucie PEDUTO
Nom du Responsable de l’Équipe : Lucie PEDUTO
INSTITUT PASTEUR, 25 rue du Docteur Roux, 75015 Paris